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stemmacs passaging solution xf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec stemmacs passaging solution xf
    Stemmacs Passaging Solution Xf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemmacs+passaging+solution+xf/StemMACS+Passaging+Solution+XF%2C+human/us12686853-568-8-12
    Average 95 stars, based on 35 article reviews
    stemmacs passaging solution xf - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Passaging:

    Article Title: Oropouche virus infects human neural progenitor cells and alters the growth of brain organoids
    Article Snippet: StemMACS TM iPS-Brew XF , Miltenyi Biotec , Cat# 130-104-368. .. StemMACS Passaging Solution XF , Miltenyi Biotec , Cat# 130-104-688. ..

    Article Title: Methods and compositions for treatment of muscle disease with iPSC-induced human skeletal muscle stem cells
    Article Snippet: Isolated iPSC clones were confirmed to be OCT4 positive and Sendai virus negative. iPSCs were routinely maintained on Matrigel® (Corning) with StemMACS iPS-Brew XF® (Miltenyi Biotec). .. The cells were passaged every 4 days using StemMACS Passaging Solution XF® (Miltenyi Biotec) and the Rock inhibitor Y27632 (10 μM, STEMCELL Technologies) for 24 hr to improve the survival rates. .. iPSC typically plated on 6-well plates, were induced for myogenic differentiation using media prepared by Genea Biocells and now commercially available as a skeletal muscle differentiation kit with SKM03 S3 Stage differentiation medium (Myocea) following the manufacturer's specifications.

    Article Title: Cytosine base editing workflow for quality-controlled multiplex-knockout hiPSC lines
    Article Snippet: .. For hiPSCs, on the third day after the last passaging or when the hiPSCs reached more than 90% confluency, cells were seeded onto a 6-well plate for transfection (300,000 cells per well) using StemMACS Passaging Solution XF (Miltenyi Biotec). .. During seeding, 2.5 μM StemMACS Y-27632 (Miltenyi Biotec) was added until proceeding with the transfection.

    Article Title: ABE9 fused to SpRY Cas9 nickase enables precise generation of bystander free mouse models.
    Article Snippet: The hiPSCs were cultured on hESC-Qualified Matrix Matrigel-coated (Corning) 6-well plates (Sarstedt) in StemMACS PSC-Brew XF medium (Miltenyi Biotec). .. Medium was changed daily, and cells were passaged every three days using StemMACS Passaging Solution XF (Miltenyi Biotec). .. The culture medium was supplemented with 2.5 μM Rock Inhibitor (Y27632, Miltenyi Biotec) on passaging days for 24 hours.

    Article Title: Non-destructive transcriptomics via vesicular export
    Article Snippet: .. At ~70% confluence, hiPSCs were passaged using StemMACS Passaging Solution XF (Miltenyi Biotec) or Accutase (Gibco) at room temperature for 5 min, followed by manual dissociation in E8 Flex medium. .. Murine primary neurons (Gibco Thermo Fisher Scientific, A15586) were recovered according to the manufacturer’s protocol and maintained on PDL-coated (100 μg/mL) plates.

    Article Title: ABE9 fused to SpRY Cas9 nickase enables precise generation of bystander free mouse models
    Article Snippet: The hiPSCs were cultured on hESC-Qualified Matrix Matrigel-coated (Corning) 6-well plates (Sarstedt) in StemMACS PSC-Brew XF medium (Miltenyi Biotec). .. Medium was changed daily, and cells were passaged every three days using StemMACS Passaging Solution XF (Miltenyi Biotec). .. The culture medium was supplemented with 2.5 μM Rock Inhibitor (Y27632, Miltenyi Biotec) on passaging days for 24 h. The supernatant media from cell cultures were routinely analysed for mycoplasma presence using the MycoSPY Master Mix (Biontex).

    Article Title: CRISPR/Cas9 Screens Implicate RARA and SPNS1 in Doxorubicin Cardiotoxicity.
    Article Snippet: Induced pluripotent stem cells (iPSCs) were maintained in Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec). .. Cells were passaged using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz) to improve survival. iPSCs were differentiated into iCMs using the Wnt/β-catenin modulation protocol, 9 with matrix overlay to enhance epithelial-to-mesenchymal transition 10 and lactate selection to promote maturation. ..

    Transfection:

    Article Title: Cytosine base editing workflow for quality-controlled multiplex-knockout hiPSC lines
    Article Snippet: .. For hiPSCs, on the third day after the last passaging or when the hiPSCs reached more than 90% confluency, cells were seeded onto a 6-well plate for transfection (300,000 cells per well) using StemMACS Passaging Solution XF (Miltenyi Biotec). .. During seeding, 2.5 μM StemMACS Y-27632 (Miltenyi Biotec) was added until proceeding with the transfection.

    other:

    Article Title: Oropouche virus infects human neural progenitor cells and alters the growth of brain organoids
    Article Snippet: Hamster BSR-T7/5 cells (Baby hamster kidney fibrobalst; gift of Alain Kohl, MRC-University of Glasgow, Scotland), which stably express the T7 RNA polymerase, were grown in GMEM supplemented with 10% (FBS), 1% P/S, 10% tryptose phosphate broth (Thermo Fisher Scientific), 1% GlutaMAX and Geniticin (1 mg/mL).

    Selection:

    Article Title: CRISPR/Cas9 Screens Implicate RARA and SPNS1 in Doxorubicin Cardiotoxicity.
    Article Snippet: Induced pluripotent stem cells (iPSCs) were maintained in Geltrex-coated 6-well plates using StemMACS iPS-Brew XF media (Miltenyi Biotec). .. Cells were passaged using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz) to improve survival. iPSCs were differentiated into iCMs using the Wnt/β-catenin modulation protocol, 9 with matrix overlay to enhance epithelial-to-mesenchymal transition 10 and lactate selection to promote maturation. ..



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    95
    Miltenyi Biotec stemmacs passaging solution xf
    Stemmacs Passaging Solution Xf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemmacs+passaging+solution+xf/StemMACS+Passaging+Solution+XF%2C+human/us12686853-568-8-12
    Average 95 stars, based on 1 article reviews
    stemmacs passaging solution xf - by Bioz Stars, 2026-11
    95/100 stars
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